Figure 5. Complementing C. briggsae and C. remanei with Cel-sid-2.

(A) Sensitivity to ingested Oti-actin RNAi of two C. briggsae integrated transgenic lines, JU1018 and JU1076, transformed with Cel-sid-2 genomic DNA and a myo-2::DsRed2 marker. Both lines are rendered sensitive to external actin dsRNA application, compared to the reference strain AF16. JU1018 has a lower brood size than the other lines. Statistical comparisons were made to the results of control experiments with Cel-rol-6 or Cbr-lin-12 dsRNA using a Mann-Whitney-Wilcoxon rank sum test on the number of normal larval progeny of each parent. Note that this test is very conservative and has low power. The significance of the difference is depicted as follows: (NS) non-significant, (*) 0.01<p<0.05, (**) 0.001<p<0.01, (***) p<0.001. Error bars indicate the standard error of the mean over individuals (n = 6–12, see Table S1 for details). (B) Sensitivity to Cre-unc-22 RNAi by feeding of a C. remanei transgenic line, JU1184, transformed with Cel-sid-2 genomic DNA and a myo-2::DsRed2 marker. The transgenic strain C. remanei JU1184 displayed the characteristic twitching phenotype when Cre-unc-22 dsRNAs were administered by feeding, whereas no twitcher was seen in the reference strain C. remanei PB4641. χ² test: p = 10⁻⁷⁵.