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. 2012 Jan 11;7(1):e29735. doi: 10.1371/journal.pone.0029735

Figure 1. Incubation with dbcAMP or IBMX disrupts asymmetric division in meiosis I in mouse oocytes.

Figure 1

Denuded mouse oocytes were cultured in DMSO control medium for 16 hours (control group), or in M16 medium containing 0.3 mM dbcAMP or 0.2 mM IBMX for 24 hours, followed by 16 hours of culture in M16 medium (dbcAMP and IBMX group). (A) Representative images of different oocyte phenotypes after incubation. GV designates oocytes remaining in the GV stage; 1Pb designates mature eggs that extruded a first polar body in the first meiotic division; 1-cell designates oocytes that displayed only one cell; 2-cell designates oocytes that produced two daughter cells of similar sizes in the first meiotic division. Arrowheads are used to indicate 2-cell oocytes. Scale Bar: 100 µm. (B) Comparison of cell division types between oocytes treated with or without IBMX or dbcAMP. Data are the mean ± SEM from 3 independent experiments. P values were calculated with a 2×4 χ2-test. N: number of cells analyzed.