Figure 2. High intracellular cAMP disrupts asymmetric division in post-GVBD oocytes, and H-89 rescues this effect on asymmetric division.

Denuded mouse oocytes at the GV stage were collected and cultured in M16 medium for 90 min. Then, oocytes that had undergone GVBD were transferred into DMSO control medium or medium supplemented with different drugs and cultured for 16 hours. (A) Representative images of oocytes after a 16 hour incubation. Arrowheads indicate 2-cell oocytes. Scale bar: 100 µm. (B) Oocyte division results after 16 hours incubation. Data are the mean ±SEM from 3 independent experiments. ** P<0.01, 2×3 χ2-test, compared to concurrent control.