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. 2012 Jan 11;7(1):e29735. doi: 10.1371/journal.pone.0029735

Figure 4. Cortical specification in the primary oocyte is dependent on chromosome cluster position.

Figure 4

Representative images of oocytes before cytokinesis initiation (A) and after cytokinesis completion (B). Denuded oocytes were incubated in the presence of 0.3 mM dbcAMP or 0.2 mM IBMX for 24 hours, followed by incubation in M16 for 9 hours, or in control medium in the absence of dbcAMP or IBMX for 10 hours, and subsequently fixed and co-stained with Alexa594-phalloidin (red) and Hoechst 33342 (blue). Oocytes with (row 1 in panel A) or without (row 2 in panel A) an “F-actin cap” were observed in both the control group and treatment groups. Arrowheads indicate “F-actin cap” localization. Scale bar: 20 µm.