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. 2012 Jan 11;7(1):e29735. doi: 10.1371/journal.pone.0029735

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Chen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunostaining of control (CTRL) oocytes cultured in control medium for 10 hours and oocytes cultured in M16 medium for 10 hours after a 20 hour incubation with 0.3 mM dbcAMP or 0.2 mM IBMX. Oocytes were stained with anti-phospho-MLC2 (Ser19) antibody (myosin II, green) and Hoechst 33342 (DNA, blue). (B) The myosin II activities of control or treated oocytes were assayed. Extracts from control, dbcAMP-treated and IBMX-treated oocytes were immunoblotted with antibodies against phospho-MLC2 or β actin. Each lane represents a protein sample derived from 300 oocytes.