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. 2012 Jan 11;7(1):e30052. doi: 10.1371/journal.pone.0030052

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Mosco et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Reverse Phase HPLC profiles of the purified refolded synthetic polypeptides and their corresponding ESI-MS multiply charged ion and deconvoluted spectra (insets): L-Phe³-cHH (A and B), D-Phe³-cHH (C and D), Glp-D-Phe³-cHH (E and F). Mobile Phase A: 0.1% TFA in water. Mobile Phase B: 0.1% TFA in MeCN. Gradient: 0–100% B over 25 min. Column: Gemini 5 C18 2.0×150 mm.