Figure 1. Deletion of profilin1 in Pfn1^{flx/flx,CaMKII-cre} mice.

(A) Immunoblot analysis in different brain regions from an adult Pfn1^flx/flx control and an adult Pfn1^{flx/flx,CaMKII-cre} mutant (P70), revealing efficient deletion of profilin1 in the forebrain of mutants. In all three forebrain tissues (cortex (CX), striatum (STR), hippocampus (HIP)), profilin1 expression was almost undetectable in mutants. In contrast, profilin1 expression level was unchanged in the cerebellum (CB), in which cre is not expressed. Identical results were obtained when investigating profilin1 expression levels in two other Pfn1^{flx/flx,CaMKII-cre} mice. (B) Immunoblot analysis of hippocampal synaptosomes, demonstrating the absence of profilin1 from synaptic structures in mutants. (C) No changes in profilin2 expression were found in the cortex, hippocampus, or striatum of three individual profilin1-deficient mice. (D) Normal profilin2 content in hippocampal synaptosomes from two individual Pfn1^{flx/flx,CaMKII-cre} mice. Expression of β tubulin was examined to control protein load in A–D.