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. 2012 Jan 11;7(1):e30054. doi: 10.1371/journal.pone.0030054

Figure 2. Relative transcriptional activity of selected HERV taxa in human brain cell lines.

Figure 2

Cells were treated with (A) 5 mM VPA and (B) 10 µM haloperidol, risperidone, or clozapine, and compared to untreated cells. QRT-PCR experiments were performed on RNA samples that were obtained from two independent treatments with VPA, haloperidol, risperidone, or clozapine previously used for the retrovirus-specific microarray. Relative transcription levels were quantified according to Pfaffl et al. [41]. For amplification of HERV-K(HML-2) transcripts primers derived from the gag region were used. Transcription of other HERV subgroups was analyzed using pol specific primers overlapping with the capture probes of the microarray. The level of HERV transcripts was normalized to RPII and represents the mean value of at least triplicate qRT-PCR experiments. Numbers on the Y-axis show the fold up-regulation. A less than 2.5-fold increase of transcript levels is not considered as a significant transcriptional activation.