Table 1. Primers used for preparation of the vav1 promoter constructs*.
Construct | Primer name | Sequence (5′->3′) |
Le2 (Sense) | lil30 | AAGAGCTCGAAGTGGGTGAATTCTGGG |
Le2 (Antisense) | lil32 | AACTCGAGCTGGGACATCTGGGGC |
Le7 (Sense) | lil40 | CAGGCAAAGAAGAGGAAG |
Le7 (Antisense) | lil41 | TTTCTGTCGCCCTGAGAG |
Le12 (Sense) | lil38 | CAGGCAAAGAAGAGGAAG |
Le12 (Antisense) | lil39 | TAACTGGTGCCCTGAGAGG |
Le13 (Sense) | lil59 | GAAAAAGTGGTAGCACTAGCTGTC |
Le13 (Antisense) | lil60 | TGAGAGGGGGTGGAGGA |
Le15 (Sense) | lil69 | GAAAAAGTGGTAGCACTAGCTGTC |
Le15 (Antisense) | lil70 | TTCTTTGCCTGTAACTGTCG |
Le17 (Sense) | lil57 | GTAGCACTAGCTGTCGC |
Le17 (Antisense) | lil58 | CTGTAACTGTCGCCCTGA |
Le19 (Sense) | lil71 | GCAAAGAAGAGGAAGTGGT |
Le19 (Antisense) | lil72 | CTGTAACAATCGCCCTGAG |
Le20 (Sense) | lil75 | GAAAGAGATGTCAGATTCTG |
Le20 (Antisense) | lil76 | CTCGACACGGCCTG |
*The underlined sequences correspond to the nucleotide replacement mutations.