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. 2012 Jan 11;7(1):e29939. doi: 10.1371/journal.pone.0029939

Table 2. Transfection conditions for different cell lines used in this study.

Cell line Plate Density Total volume (ml) Transfection reagent Le2 (µg) Renilla (ng)
Jurkat 12 well 5×105/ml 4 Electroporation (BioRad, USA) 2 20
U937 24 well 5×105/ml 1 Lipofectamine 2000 (Invitrogen,USA) 2 20
H441 6 well 5×105/well 4 JetPEI (Polyplus, France) 2 20
H460 6 well 2×105/well 4 JetPEI (Polyplus, France) 2 20
Panc1 6 well 105/well 4 JetPEI (Polyplus, France) 2 20
A549 6 well 105/well 4 JetPEI (Polyplus, France) 2 20