Skip to main content
. 2012 Jan 11;7(1):e29939. doi: 10.1371/journal.pone.0029939

Table 3. Oligonucleotides used in EMSA, introduced mutations are underlined.

Description Oligonucleotides Sense (5′->3′) Antisense (5′->3′)
E2F/NF-e/c-Myb and TCF μ/PU.1/ELF1 binding sites (−45 to 0) lil157-158 CCCTCTCAGGGCGACATTACAGGCAAAGAAGAGGAAGTGGTAGC GCTACCACTTCCTCTTCTTTGCCTGTAACTGTCGCCCTGAGAGGG
−27–28 TT>AA substitution (−45 to 0) lil159-160 CCCTCTCAGGGCGACAGAAACAGGCAAAGAAGAGGAAGTGGTAGC GCTACCACTTCCTCTTCTTTGCCTGTTTCTGTCGCCCTGAGAGGG
−32–33 GA>AC substitution (−45 to 0) lil161-162 CCCTCTCAGGGCACCAGTTACAGGCAAAGAAGAGGAAGTGGTAGC GCTACCACTTCCTCTTCTTTGCCTGTAACTGGTGCCCTGAGAGGG
E2F/NF-e/c-Myb binding site (−39 to −22) lil87-88 CAGGGCGACAGTTACAGG CCTGTAACTGTCGCCCTG
−27–28 TT>AA substitution (−39 to −22) lil89-90 CAGGGCGACAGAAACAGG CCTGTTTCTGTCGCCCTG
−32–33 GA>AC substitution (−39 to −22) lil147-148 CAGGGCACCAGTTACAGG CCTGTAACTGGTGCCCTG
Sp1 binding site (−160 to −141) lil79- 80 GTGTCGAGTGGGCGGAAGAA TTCTTCCGCCCACTCGACAC
CpG 3+4 methylated (−160 to −141) lil85-86 GTGTmetCGAGTGGGmetCGGAAGAA TTCTTmetCCGCCCACTmetCGACAC
CpG3 methylated (−160 to −141) lil81-84 GTGTmetCGAGTGGGCGGAAGAA TTCTTCCGCCCACTmetCGACAC
CpG4 methylated (−160 to −141) lil82-83 GTGTCGAGTGGGmetCGGAAGAA TTCTTCmetCGCCCACTCGACAC