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. 2012 Jan 11;7(1):e29897. doi: 10.1371/journal.pone.0029897

Figure 3. Capa, Neuromedin and Hugin receptor (CG8795)-associated calcium signatures.

Figure 3

(A) Typical cytoplasmic Ca2+ response in S2 cells expressing capaR and apoaequorin challenged with Drm-capa-1, Drm-PK-1, hugg, Drm-PK-2 and NMU-25 at a concentration of 10−7 M. (B) Typical cytoplasmic Ca2+ response in S2 cells expressing NMUR 2 and apoaequorin challenged with Drm-capa-1, Drm-PK-1, hugg, Drm-PK-2 and NMU-25 at a concentration of 10−7 M. (C) Typical cytoplasmic Ca2+ response in S2 cells expressing CG8795and apoaequorin challenged with Drm-capa-1, Drm-PK-1, hugg, Drm-PK-2 and NMU-25 at a concentration of 10−7 M. (D) Human NMU-25 dose-response curve in S2 cells and intact tubule. NMU-25 peptide stimulation of NMUR 2- or capaR- and apoaequorin-co-transfected S2 cells; and of tubule principal cells expressing apoaequorin transgene. Cells or tubules were challenged with increasing concentrations of agonist, and [Ca2+]i was measured. Values were expressed as maximal (nM) - background (nM) (mean ± S.E.M., N = 3).