Figure 5. Immunocytochemical localization of the capa-expressing neurons with a vertebrate NMU antibody.

Larval central nervous system was doubly labeled using an antibody against capa precursor (A, red), an antibody against porcine neuromedinU-8 (B, green). The immunoreactive cells bodies and neurohemal organs, the retrocerebral complex for the 2 cell bodies in the subesophageal neuromere, and the abdominal median transverse nerves for the 3 pairs of abdominal neuroendocrine cells all co-localize (C, yellow, merge). Scale bar 100 µm.