Figure 3. RT-PCR analysis of the expression of the genes encoding junctional proteins and of the choroid plexus markers Transthyretin, IGF2 and FOXJ1 in HIBCPP.

HIBCPP were grown in 6well plates until confluency and subsequently cultured for 1 day in medium containing 15, 1 or 0% FCS as indicated at the top of the lanes. The expression of the genes indicated at the right was analysed by RT-PCR. For comparison, RNA isolated from HeLa and Jurkat cells was analysed as well. Expression of the GAPDH gene served as control. The results shown are a typical example from three independently performed experiments.