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. 2012 Jan 11;7(1):e30069. doi: 10.1371/journal.pone.0030069

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Schwerk et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Invasion of S. suis and N. meningitidis strains was analysed by double immunofluorescence. Standard (white bars) and inverted (black bars) transwell filter cultures were infected with the indicated strains for 4 h before immunofluorescence staining and quantification of invasion as described in Experimental procedures. Data shown are the mean+SD of a minimum of four independent experiments each performed at least in duplicates. ** (highly significant; p<0.01), *** (extremely significant; p<0.001); when invasion in the inverted filter system compared to invasion in the standard filter system. # (significant; p<0.05), ### (extremely significant; p<0.001); when invasion of the unencapsulated strain compared to invasion of the capsulated strain in the standard or inverted filter system, respectively. §§ (highly significant; p<0.01); when invasion of the Opc complemented strain compared to the respective not complemented strain.