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. 2012 Jan 11;7(1):e30145. doi: 10.1371/journal.pone.0030145

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Ho et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Whole-cell protein extracts from HEp-2 cells analyzed by immunoblotting showed that (A) IFNγ mediated (50ng/ml, 0.5h) Stat-1 tyrosine phosphorylation is suppressed by enterohemorrhagic Escherichia coli O157:H7, strain EDL933 (EHEC), but not by enteropathogenic Escherichia coli O127:H6, strain E2348/69 (EPEC) (MOI of 100∶1; 6h). (B) Incubation with sterile culture supernatants (6h) from EHEC, but not EPEC, suppressed IFNγ-mediated tyrosine phosphorylation of Stat-1. (C) Incubation with chloramphenicol (100 µg/ml) up to 1h after infection with EHEC (MOI 100∶1; 6h) prevented EHEC suppression of IFNγ mediated Stat-1 tyrosine phosphorylation.