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. 2012 Jan 11;7(1):e30145. doi: 10.1371/journal.pone.0030145

Figure 5. Purified YodA failed to inhibit Stat-1 tyrosine phosphorylation as well as wild-type culture supernatant.

Figure 5

EHEC yodA gene was cloned into the pBAD protein expression vector (A) and verified by PCR (B). Over-expressed YodA-His was detected in the soluble fraction, but not in the insoluble or supernatant fraction, by western blot (C) and purified using nickel column chromatography (D). Varying concentrations of eluted and dialyzed YodA-His were tested for the ability to inhibit IFNγ mediated Stat-1 tyrosine phosphorylation (panels E and F, respectively).