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FRET behavior in mixed micelle formulations upon excitation of donor fluorophore. Donor fluorophore (Alexa Fluor® 488, peak emission at 519 nm) was conjugated to PEO-PHB-PEO, and acceptor fluorophore (Alexa Fluor® 555, peak emission at 565 nm) was conjugated to PF-127. Increasing fraction of PF-127 correlates with decreased ratio of donor/acceptor fluorescence when excited at donor wavelength (488 nm), indicating colocalization of donor/acceptor fluorophores.
