Table 1.

Description of strains and plasmids used in this study

Strain or Plasmid	Description & Purpose	Reference
RK103	Δccm E.coli strain deleted for all ccm genes	[28]
RK106	ΔccmΔdsbA E. coli strain Generated by P1 phage transduction using strains JW3832 and RK103	This study
RK107	ΔccmΔdsbB E. coli strain Generated by P1 phage transduction using strains JW5182 and RK103	This study
RK108	ΔccmΔdsbC E. coli strain Generated by P1 phage transduction using strains JW2861 and RK103	This study
RK109	ΔccmΔdsbD E. coli strain Generated by P1 phage transduction using strains JW5734 and RK103	This study
RK110	ΔccmΔdsbG E. coli strain Generated by P1 phage transduction using strains JW0597 and RK103	This study
RK112	Δccm BL21(DE3) E. coli stain Generated by P1 transduction with RK103	This study
TC127034	cDNA clone from Origene-plasmid template for construction of pRGK390 and pRGK391	Origene
MGC12367	E. coli strain from ATCC with plasmid PCR template for construction of pRGK392	ATCC
pRGK333	Expression of GST-tagged CcmABCDEFGH (system I) from E. coli	[28]
pRGK368	Expression of GST-tagged CcsBA (fused system II) from H. hepaticus	[29]
pRGK330	pBAD24 based, chloramphenicol resistant plasmid for arabinose inducible expression	[28]
pRGK399	Periplasmic expression MBP-tagged human cytochrome c heme lyase (CCHL; system III)	This study
pRGK400	Periplasmic (pelB signal) of N-terminal 6x: His-tagged human CCHL	This study
pRGK331	Expression of cytochrome c4 from B. pertussis fused to alkaline phosphatase. PCR template for construction of pRGK392	[28]
pRGK332	Expression of cytochrome c4 reporter from B. pertussis	[28]
pRGK389	Expression of cytochrome c2 reporter from R. capsulatus	This study
pRGK390	Periplasmic (cyt c4 signal) expression of human cytochrome c CycS reporter	This study
pRGK401	Periplasmic (cyt c4 signal) expression of chimeric human CycS and R. capsulatus cytochrome c2 reporter	This study