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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Mol Microbiol. 2011 Dec 11;83(2):319–334. doi: 10.1111/j.1365-2958.2011.07932.x

Figure 8.

Figure 8

Quantitative PCR shows a lowered infectious load for the bb0646 mutant at a low inoculum dose. Quantitative PCR was performed on (A) joint (B) lymph node and (C) skin tissues removed from mice that were infected with 103 organisms to determine the absolute number of B. burgdorferi genomic equivalents in each tissue sample. Copies of recA were quantified and represent the number of total B. burgdorferi genomes. To normalize the data to host tissue, copies of β-actin were also enumerated and data points are expressed as copies of B. burgdorferi genomes per 106 copies of β-actin. Bars indicate average value of the sample tested. The asterisks denote a P value < 0.05.