| E. coli strains |
| Rossetta (DE3) pLysS |
CamR; F− ompT hsdSB(rB− mB−) gal dcm λ(DE3) pLysSRARE |
Novagen |
| Mach-1™-T1R
|
ϕ80lacZΔM15 ΔlacX74 hsdR (rK−, mk+) ΔrecA1498 endA1 tonA
|
Invitrogen |
| B. burgdorferi strains |
| ML23 |
B. burgdorferi strain B31 clonal isolate missing lp25 |
(Labandeira-Rey and Skare, 2001) |
| DS102 |
ML23, bb0646:gentR
|
(Hyde et al., 2010) |
| ML23/pBBE22 |
Missing lp25 complemented with pBBE22 |
(Seshu et al., 2006) |
| DS102/pBBE22 |
ML23, bb0646::gentR complemented with pBBE22 |
This study |
| DS102/pDS113 |
ML23, bb0646::gentR complemented with pBBE22 containing PbosR-bb0646
|
This study |
| DS102/pDS126 |
ML23, bb0646::gentR complemented with pBBE22 containing PflgB-bb0646
|
This study |
| DS102/pDS127 |
ML23, bb0646::gentR complemented with pBBE22 containing PflgB-bb0646-S152A
|
This study |
| DS102/pDS128 |
ML23, bb0646::gentR complemented with pBBE22 containing PflgB-bb0646-S152T
|
This study |
| Plasmids |
| pCR8/GW/TOPO |
SpecR; Gateway PCR cloning/entry vector |
Invitrogen |
| pCR2.1Bactin |
KanR; β-actin gene cloned into pCR2.1 vector |
(Maruskova et al., 2008) |
| pCR2.1recA
|
KanR; 1119 bp fragment, containing the recA gene, in pCR2.1 |
This study |
| pET15b |
CarbR; PT7lac lacI bla His6 coding sequence (5’) pBR322 origin |
Novagen |
| pBSV2 |
KanR; borrelial shuttle vector |
(Stewart et al., 2001) |
| pBSV2G |
GentR; borrelial shuttle vector |
(Elias et al., 2003) |
| pBBE22 |
KanR; borrelial shuttle vector carrying bbe22
|
(Purser et al., 2003) |
| pBBE22Gate |
CamR, KanR; pBBE22 modified to be a Gateway destination vector containing attL and attR sites |
(Weening et al., 2008) |
| pDS200 |
SpecR; bb0646 without the coding region of the putative leader peptide (bp 1–63) cloned into pCR8/GW/TOPO |
This study |
| pDS201 |
CarbR; bb0646 without the coding region of the putative leader peptide (bp 1–63) cloned into pET15b |
This study |
| pDS110 |
SpecR; 440 bp fragment of the bosR/bb0646 promoter region (PbosR) cloned into pCR/GW/TOPO |
This study |
| pDS111 |
SpecR; full-length bb0646 cloned into pCR/GW/TOPO |
This study |
| pDS112 |
SpecR; full-length bb0646 cloned from pDS111 into pDS110 to yield a PbosR-bb0646 transcriptional fusion |
This study |
| pDS113 |
KanR; pDS112 recombined into pBBE22Gate via the Gateway system |
This study |
| pDS120 |
SpecR; 535 bp of the flagellar promoter (PflgB) amplified from pBSV2 and cloned into pCR8/GW/TOPO |
This study |
| pDS122 |
SpecR; full-length bb0646 cloned from pDS111 into pDS120 to yield a PflgB-bb0646 transcriptional fusion |
This study |
| pDS126 |
KanR; pDS122 recombined into pBBE22Gate via the Gateway system |
This study |
| pDS127 |
KanR; pDS126 containing the PflgB-bb0646-S152A allele |
This study |
| pDS128 |
KanR; pDS126 containing the PflgB-bb0646-S152T allele |
This study |
