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Comet-to-plaque transition using agarose dilutions. Low MOI influenza virus infections were incubated under varying agarose concentrations for 40 hours under “wet” (100% relative humidity) or “dry” (20% relative humidity) conditions. The infections took on either a “comet” or “plaque” morphology with a sharp transition between 0.05% and 0.1% agarose.
