Figure 4.

Wee1 expression prevents hydrogen peroxide-induced cellular injury. (A) Number of cells after Wee1 expression under hydrogen peroxide. Cells were transiently transfected with either the wild-type or mutant Wee1 and then maintained for 24 h, prior to hydrogen peroxide stimulation. Cells were exposured to hydrogen peroxide for 24 h with indicated doses. The results are expressed as the mean ± SEM for three separate experiments. (B) Morphologic changes of MS-1 cells after Wee1 expression. Cells were transiently transfected with either Green Fluorescent Protein-tagged wild type or mutant Wee1, prior to hydrogen peroxide exposure. Images were obtained with confocal microscopy. Green color indicates GFP-transfected cell, red color indicate non-transfected cell.