Fig. 7. PGE2 transport.
H358 WT cells treated with 1 mM probenecid for 24 h, and H358 cells transduced with shMRP4 were treated with 10 μM AA for 4 h. After AA treatment for 4 h, 3 mL of media was taken and lipids were extracted for PGE2 analysis by chiral normal phase LC-ECAPCI/SRM/MS as described previously (Lee and Blair, 2007). (*) indicates p<0.05 compared to control and (**) indicates p<0.05 compared to control; n=3.