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. 2011 Sep 1;8(5):771–781. doi: 10.4161/rna.8.6.16014

Figure 2.

Figure 2

GluRIIA and GluRIIB mRNA expression in scratch mutant embryos. (A) Confocal micrograph of motor neuron terminals from intersegmental (ISN) nerve branch ISNb innervating ventral longitudinal muscles 15, 6/7, 13 & 12 in control (w[1118]) embryos (24 h AEL). Motor neuron terminals were visualized using fluorescently-conjugated anti-HRP antibodies. (B) Confocal micrograph as in (A), but showing ISNb in a scratch[KG02164] mutant embryo, where body wall NMJs do not form. Scale bar: 5 um. (C) GluRIIA mRNA abundance measured from scratch[KG02164] mutant embryos, using quantitative real time RT-PCR, as described for Figure 1A. (D) GluRIIB mRNA abundance measured from scratch[KG02164] mutant embryos, using quantitative real time RT-PC R, as described for Figure 1B. Note the lack of GluRIIA or GluRIIB mRNA expression.