Figure 1.

Colletotrichum truncatum biotrophy-necrotrophy switch library. (A) Trypan blue-stained in planta (Lens culinaris Medik. cv. Eston) fungal structures of C. truncatum. Excized lentil leaflets were droplet-inoculated with conidial suspension (1 × 10⁴ conidia/ml) of C. truncatum and harvested at 48 h post-inoculation. BH, biotrophic hyphae; BNS, biotrophy-necrotrophy switch; NH, necrotrophic hyphae. Bar = 10 µM. (B) A directional cDNA library was constructed from mRNA isolated from lentil leaflet tissues displaying the switch from fat BH to thin NH and 5,000 ESTs were generated. Proportion of fungal and plant ESTs is illustrated. (C) One hundred sixty-two ESTs (assembled into 122 unique sequences or unigenes) were likely to encode proteins with putative signal peptide sequence at their N-terminus. Pie chart shows potential functional groups identified from unigenes encoding putative secretory proteins. CEAPs, cell envelope-associated proteins.