Effects of acetylcholine (ACh) on the elongation of stems and roots of radish seedlings. The seedlings were cultured in the presence and absence of acetylcholine (0, 0.01, 0.1, 1, 10 and 100 nM) for 5 d at 25°C in the dark. The lengths of the stem, the main (primary) root and total lateral roots per radish seedling at day 5 are shown. Vertical bars represent the means ± SD (n = 15).