Figure 9.

Effects of ACh on DNA content in roots (main + lateral roots) of radish seedlings. The seedlings were cultured in the presence and absence of ACh (1 nM) for 5 d at 25°C in the dark. (A) DNA fraction was obtained after the treatment with RNase. DNA content was estimated at A₂₆₀. The ratio of A₂₆₀/A₂₈₀ in the roots of the seedlings cultured in the presence of ACh was 1.49 and that in the absence of ACh 1.51. Vertical bars represent the means ± SE of results from triplicate experiments. The asterisk indicates a significant difference between in the absence and presence of ACh at p < 0.01 by the t-test (n = 3). (B) Agarose gel eclectrophoresis image of the DNA content in DNA fractions. The electrophoresis was preformed using respective aliquots of the DNA fractions obtained in (A).