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. 2011 Oct 1;6(10):1554–1563. doi: 10.4161/psb.6.10.17443

Figure 7.

Figure 7

Identification of indole-3-carboxaldehyde from underivatized samples from T. virens growth medium by GC-MS. (A) chromatogram from neutral ethyl acetate extract obtained from 1 l of culture medium of T. virens, arrows indicate the presence of different indolic compounds, indole-3-acetaldehyde (IAAld), indole-3-ethanol (IEt) and indole-3-carboxaldehyde (ICAld). (B) ICAld standard. (C) the 70-eV electron-impact full-scan mass spectra from m/z 50 to 500 of ICAld standard. (D) Indole-3-carboxaldehyde increases camalexin biosynthesis. Induction of camalexin in shoots of Arabidopsis after treatment with 300 µM ICAld for 72 h. Bars shown in (D) represent the mean ± SD of three independent replicates. Each replicate included 20 seedlings. Different letters are used to indicate means that differ significantly (p < 0.05). The experiment was repeated twice with similar results.