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. 2011 Oct 1;6(10):1616–1619. doi: 10.4161/psb.6.10.16981

Figure 2.

Figure 2

Conditions of LCBs elevation produce H2O2 formation in the chloroplast and perturbation in the membrane morphology of mitochondria. (A) Exposure of Arabidopsis leaves to the avirulent strain Pseudomonas syringae pv. tomato DC3000 (avrRPM1) (or Pst avrRPM1) induces H2O2 formation in the chloroplast. Arabidopsis leaves were infiltrated with 1 × 108 UFC/ml Pst avrRPM1 and after 18 h, samples were treated to visualize H2O2 formation with the DAB reaction. Controls were infiltrated with 10 mM MgCl2 and then processed for DAB staining. Then, samples were analyzed in an optical photomicroscope Olympus Provis Model AX70. (B) Effect of FB1 on mitochondria ultrastructure. Wild type Arabidopsis seedlings were treated with FB1 for 72 h and tissues were processed and analyzed according to Saucedo et al.2 Ch, chloroplast; M, mitochondria; PM, plasma membrane. Arrows show mitochondrial cisternae. Bars show the correspondent magnification.