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. 2011 Oct 25;20(1):204–213. doi: 10.1038/mt.2011.209

Figure 4.

Figure 4

iMSCs form bone. (a) RT-PCR of ALPL, RUNX2, and BGLAP genes in the indicated cell lines after osteogenic differentiation, with GAPDH control. (b) Alizarin red and Von Kossa staining of wild-type mesenchymal stem cells (MSCs) and iMSCs (iMSCe2-FVc2) grown with and without additional osteogenic differentiation. (c) Serial histological sections of hydroxyapatite/tricalcium phosphate matrices seeded with osteogenic iMSCe2-FVc4s and stained with Van Gieson's picric acid fuchsin, Masson's trichrome and antibodies against human bone sialoprotein and human type I collagen. Bar = 500 µm. Two high power views of the Masson's trichrome stain are included with arrow indicating osteocytes. Bar = 30 µm. (d) Serial sections of a teratoma derived from iPSCe2-FVc3 and stained as indicated showing bone formation. Bar = 100 µm. iPSCs, induced pluripotent stem cells.