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Tandem MS spectra of the light (a) and heavy (b) YQK56(ac)STELLIR peptide standards at 10 μg/mL using enhanced product ion (EPI) scan. LC-MS/MS analysis (MRM) of the native H3K56ac peptide (c) and the [13C615N1-isoleucine]-labeled YQK56(ac)STELLI*R internal peptide standard (d) from a digest of histones isolated from K562 cells treated with SAHA for 6 h.
