Figure 11.

PKC activation inhibits LIF-induced STAT3 tyrosine phosphorylation. A, Amount of tyrosine-phosphorylated STAT3 found in P1 WT retinas after 5 h explant culture followed by 30 min treatment with 100 nm PMA, 50 ng/ml IGF1, 100 nm Go7874, and 50 μm Ly294002. B, C, Amount of tyrosine phosphorylated STAT3 (B) and total STAT3 (C) found in retinas of P1 PKC-γ KO and WT littermates cultured for 5 h in the presence or absence of PCK-β1 inhibitor. D, E, Amount of tyrosine-phosphorylated STAT3 found in P1 WT retinas after 5 h explant culture followed by 3 h preincubation with either PMA (D) or IGF1 (E) with subsequent addition of LIF for 30 min. *p < 0.05; **p < 0.005; ***p < 0.0005. Error bars indicate SEM.