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. 2011 Dec 14;31(50):18606–18617. doi: 10.1523/JNEUROSCI.2578-11.2011

Figure 2.

Figure 2.

IGF1 and PMA treatment of P1 retinal explants increases expression of rod specific genes. A, B, Quantitative RT-PCR assay showing expression of opsin and Crx in P1 retinal explants cultured for 4 d in the presence of PMA (A) or IGF1 (B). *p < 0.05; **p < 0.005. Beta-actin and GAPDH expression was used to normalize mRNA levels and values are expressed relative to control levels. C, D, Amount of opsin (C) and Crx (D) proteins found in 4 d retinal explants of P1 retinas using Western blot analysis. Beta-actin expression was used to normalize total protein levels. Values in graph are normalized to control levels. *p < 0.05, **p < 0.01. Error bars indicate SEM.