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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: J Immunol. 2011 Jan 24;186(5):2765–2771. doi: 10.4049/jimmunol.1003046

FIGURE 3. Dap12 signaling required for mature NK cells to become tolerant upon transfer into m157-Tg mice.

FIGURE 3

(A) Representative dot plots demonstrating IFN-γ production by freshly isolated splenocytes stimulated with plate bound anti-NK1.1 mAb. The numbers represent the percentage of Ly49H+ or Ly49H− NK cells producing IFN-γ. The dot plots were gated on donor NK cells (NK1.1+, CD3−, CFSE+ cells). (B) The ratio of the percentage of IFN-γ producing Ly49H+ NK cells to the percentage of IFN-γ producing Ly49H− NK cells from DAP12ki→ WT (n=6), DAP12ki → m157-Tg (n=10), DAP10ko→ WT (n=4), DAP10ko → m157-Tg (n=5) mice following stimulation with plate bound anti-NK1.1 (PK136). Donor cells in (A) and (B) were assessed 7 days post transfer. The results are presented as the mean ± SEM.