Average total content of fumonisins (FB1, FB2 and FB3) in leaf samples from 14-day-old maize seedlings. Fumonisin accumulation in the first leaf of seedlings of the sweet maize line Silver Queen inoculated with wildtype (M3125 and M3120), FvVE1 deletion (M312501 and M31206), and complementation (M312501C1 and M3120C65) strains. Each treatment had three replicates of 10 seedlings, and the first leaves from seedlings were pooled per replicate. The experiment was conducted twice, and the data are reported as mean fumonisin concentration per replicate. Error bar represents standard deviation. ND, none detected. anova indicated no significant differences among the fumonisin producing strains (P = 0·183, F = 2·065, d.f. = 11).