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Purification of acetone carboxylase from cell extracts of A. aromaticuma
| Fraction | Sp act (nmol min−1 mg−1) | Purification (fold) | Yield (%) |
|---|---|---|---|
| Extract (soluble fraction) | 20.2 | 1 | 100 |
| DEAE pool | 38.6 | 2.0 | 28 |
| Gel filtration | 70.3 | 3.1 | 14 |
ATPase activity (AMP forming) was determined in a coupled photometric assay (assay II). Activities are in nmol min−1 mg protein−1.
