Fig 2.

DNase I footprint of VraR-P on the P_vraSR R1DM3 and R2SM1 variants. The top strands of P_vraSR, P_vraSR R1DM3, and P_vraSR R2SM1 were end labeled with [γ-³²P]ATP. The binding reaction mixtures consisted of VraR-P and 10 ng DNA. After treatment with DNase I, the resulting DNA fragments were separated on an 8% polyacrylamide sequencing gel containing 7 M urea. The positions of the nucleotides, as indicated by the numbers, were calibrated with respect to the transcription start position. WT, wild type.