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DNase I footprints of VraR and VraR-P on the PvraSRR1+1A, -R1+2A, and -R1+3A variants. The bottom strand of each PvraSR variant was end labeled with [γ-32P]ATP and incubated with VraR or VraR-P at 2 or 5 μM. After treatment with DNase I, the resulting DNA fragments were separated on an 8% polyacrylamide sequencing gel containing 7 M urea. The C and G sequencing reactions were calibrated with respect to the transcription start point.
