Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Runoff in vitro transcription assays with E. coli RNAP holoenzyme, using PvraSRL as a template (A), and E. coli RNAP haloenzyme, E. coli RNAP core enzyme, and S. aureus SigA in the presence of VraR-P, using PvraSRL as a template (B). The SigA concentrations in lanes 2 to 5 are 2, 4, 8, and 8 μM, respectively. The sizes of the transcription products were determined using the molecular weight marker φX174DNA/HinfI and the 160-bp DNA fragment (lane 1).
