Fig 6.

(A) Role of RNAP haloenzyme and phosphorylation of VraR in the transcription of the vraSR operon (the error bars represent the standard deviations calculated from three independent experiments). (B) Effect of VraR-P concentration on runoff in vitro transcription (error bars represent the standard deviations calculated from three independent experiments). (C) Role of intact R1 and R2 sites in transcription of the vraSR operon. The reactions were performed in the presence of VraR-P (6 μM) or VraR (6 μM). The sizes of the transcription products were determined using the φX174DNA/HinfI molecular weight marker.