Fig 7.
DNase I footprint of the RNAP holoenzyme on PvraSR in the presence or absence of VraR or VraR-P. Top strand (A) and bottom strand (B) of 32P-end-labeled PvraSR. The binding reaction mixtures included 10 ng DNA and proteins at the indicated concentrations. The regions protected by VraR-P are indicated with solid lines. The regions protected by RNAP are indicated with dashed lines. The region of DNA protected by RNAP in the presence of VraR-P is marked with a double line. The hypersensitive regions induced by RNAP are indicated by gray arrowheads, and the hypersensitive regions caused by VraR-P are indicated by black arrowheads.