Table 1.
Apparent dissociation constants of VraR and VraR-P, with PvraSR variants determined from the EMSA experimentsa
| Variant | Apparent Kd (μM) |
|
|---|---|---|
| VraR | VraR-Pb | |
| Wild type | 4.1 ± 0.5 | 1.7 ± 0.1 |
| PvraSRR1C67T | 7.1 ± 0.5 | 2.2 ± 0.4 |
| PvraSRR1C67T/G62T (R1DM3) | 9.0 ± 0.4 | 5.9 ± 0.5 |
| PvraSRR1G58T | 3.9 ± 0.5 | 3.6 ± 0.1 |
| PvraSRR1C52T | 6.1 ± 0.4 | 4.4 ± 0.3 |
| PvraSRR1G58T/C52T (R1DM4) | 7.5 ± 0.4 | 5.0 ± 0.3 |
| PvraSRR1+1A | 8.8 ± 0.4 | 4.2 ± 0.4 |
| PvraSRR1+2A | 11 ± 1.1 | 4.8 ± 0.4 |
| PvraSRR1+3A | 12.0 ± 0.6 | 5.4 ± 0.2 |
| PvraSRR2G38A (R2SM1) | 4.2 ± 0.2 | 2.8 ± 0.3 |
| PvraSRR2C43A | 5.4 ± 0.3 | 4.9 ± 0.1 |
| PvraSRR2C43A/G38A | 5.2 ± 0.3 | 4.8 ± 0.5 |
| PvraSRR2C31A | 4.2 ± 0.2 | 3.0 ± 0.1 |
| PvraSRR2G25A | 4.4 ± 0.2 | 4.0 ± 0.3 |
| PvraSRR2C31A/G25A | 5.4 ± 0.3 | 4.3 ± 0.6 |
| PvraSRR3G85T/C79T | 3.8 ± 0.3 | 2.1 ± 0.4 |
| PvraSRR3C73T/G69T | 5.4 ± 0.3 | 3.9 ± 0.6 |
a
The Hill coefficient was determined to be >1 in all the cases.
b
The phosphorylation level of VraR is 46% in these experiments.