Skip to main content
. 2012 Jan;194(1):3–14. doi: 10.1128/JB.05623-11

Table 1.

Oligonucleotide primers used in this study

Primer Sequence (5′→3′)a
virB-Tsc-For GCAAGCTTATGACAGGCATATTTCAACGCGAC
virB-Tsc-Rev GCGGATCCCGATTATTATGATAGCCTTAATTATAG
virB-Tsl-For GCGGATCCATGACAGGCATATTTCAACGCGAC
virB-Tsl-Rev GCAAGCTTCACCATAGGATCGTCTCCTTCTC
babR-Tsc-For GCAAGCTTATTATCTAATTTAGAATGAAG
babR-Tsc-Rev GCGGATCCATAAATATGAATTCTTGG
babR-Tsl-For GCGGATCCCTAATTTAGAATGAAGTTATATTC
babR-Tsl-Rev GCAAGCTTTTTCATTGCAGACATCCCAG
babR-Trunc-Up-For GCGGATCCAAAAGACCACCTTGTTCCG
babR-Trunc-Up-Rev CTTTCATCATAATTAGCGATTT
babR-Trunc-Down-For TGAAAGATATGCTAGGAATGTTT
babR-Trunc-Down-Rev GCCTGCAGACAAAGCTCAAGAGGCCGA
hfq-For CATCAATGGTGATGACTGGCCAAA
hfq-Rev CGAGCGTGGCGAAAAGCATA
Inverse-hfq-For GCCTGACGCTTTCCGATATTG
Inverse-hfq-Rev AGCCATTGTTTTTATTCCTT
hfq-comp-For AAAGCATAAGGAAGTCGTGTCG
hfq-comp-Rev CGCTTTTTTATCCTTGGGTT
16S-RT-For TCTCACGACACGAGCTGACG
16S-RT-Rev CGCAGAACCTTACCAGCCCT
virB1-RT-For TGGCAGCACCTACGCACAAG
virB1-RT-Rev GCCTTGTTCCTGACCGGGCA
babR-RT-For AAGAATTATGCGCATGACCT
babR-RT-Rev GTTCCCACCCATCTGGAAAT
babR-seq-For TTATGTCCCTGGGGCATCGAAAGAC
babR-seq-Rev AATAGACATGACATAGGATACATAC
babR-PE CGTTTCAAAATAAATATGAATTCTTGGAAT
ΔbabR-Up-For GCGGATCCGATATCCGCCTCTTTACGGA
ΔbabR-Up-Rev TTTCATTGCAGACATCCCAGTAGCGTTTC
ΔbabR-Down-For CTCTGACCTTTTCCAGTGTCCCTCGGCGG
ΔbabR-Down-Rev GCCTGCAGATCGGCAGATAAGAAGAGGA
babR-comp-For AACTGCAGGAGGAAACGGCAAAACAGCCCT
babR-comp-Rev AATGTGTAGCCTTTTTCAGGGAAAG
babR-UTR-T7-For TAATACGACTCACTATAGGGTAATCTTCGTAGAAGAAATATGAA
babR-UTR-T7-Rev TGTTTCCCATTTCATTGCAGAC
babR-20-UTR-For TAATACGACTCACTATAGGGTGAAAGATATGCTAGGAATGTTTAG
rHfq-For GCGGTCTCCGCGCATGGCTGAACGATCGCAAATCT
rHfq-Rev GCGGTCTCCTATCAGGCTTCCTCGCCTTCAAACAT
rBabR-For CATGAAATGGGAAACATTTTATG
rBabR-Rev AGGAGACCAAATGTACGGGCC
PvirB-For CTGACTGGGCGTCATTCACAC
PvirB-Rev CGTCTCCTTCTCAGAGAATGGACG
babR-RACE-Rev CAGGTCATGCGCATAATTCTTTACT
a

Underlined sections indicate restriction endonuclease recognition sequences.