Table 1.
Primer | Sequence (5′→3′)a |
---|---|
virB-Tsc-For | GCAAGCTTATGACAGGCATATTTCAACGCGAC |
virB-Tsc-Rev | GCGGATCCCGATTATTATGATAGCCTTAATTATAG |
virB-Tsl-For | GCGGATCCATGACAGGCATATTTCAACGCGAC |
virB-Tsl-Rev | GCAAGCTTCACCATAGGATCGTCTCCTTCTC |
babR-Tsc-For | GCAAGCTTATTATCTAATTTAGAATGAAG |
babR-Tsc-Rev | GCGGATCCATAAATATGAATTCTTGG |
babR-Tsl-For | GCGGATCCCTAATTTAGAATGAAGTTATATTC |
babR-Tsl-Rev | GCAAGCTTTTTCATTGCAGACATCCCAG |
babR-Trunc-Up-For | GCGGATCCAAAAGACCACCTTGTTCCG |
babR-Trunc-Up-Rev | CTTTCATCATAATTAGCGATTT |
babR-Trunc-Down-For | TGAAAGATATGCTAGGAATGTTT |
babR-Trunc-Down-Rev | GCCTGCAGACAAAGCTCAAGAGGCCGA |
hfq-For | CATCAATGGTGATGACTGGCCAAA |
hfq-Rev | CGAGCGTGGCGAAAAGCATA |
Inverse-hfq-For | GCCTGACGCTTTCCGATATTG |
Inverse-hfq-Rev | AGCCATTGTTTTTATTCCTT |
hfq-comp-For | AAAGCATAAGGAAGTCGTGTCG |
hfq-comp-Rev | CGCTTTTTTATCCTTGGGTT |
16S-RT-For | TCTCACGACACGAGCTGACG |
16S-RT-Rev | CGCAGAACCTTACCAGCCCT |
virB1-RT-For | TGGCAGCACCTACGCACAAG |
virB1-RT-Rev | GCCTTGTTCCTGACCGGGCA |
babR-RT-For | AAGAATTATGCGCATGACCT |
babR-RT-Rev | GTTCCCACCCATCTGGAAAT |
babR-seq-For | TTATGTCCCTGGGGCATCGAAAGAC |
babR-seq-Rev | AATAGACATGACATAGGATACATAC |
babR-PE | CGTTTCAAAATAAATATGAATTCTTGGAAT |
ΔbabR-Up-For | GCGGATCCGATATCCGCCTCTTTACGGA |
ΔbabR-Up-Rev | TTTCATTGCAGACATCCCAGTAGCGTTTC |
ΔbabR-Down-For | CTCTGACCTTTTCCAGTGTCCCTCGGCGG |
ΔbabR-Down-Rev | GCCTGCAGATCGGCAGATAAGAAGAGGA |
babR-comp-For | AACTGCAGGAGGAAACGGCAAAACAGCCCT |
babR-comp-Rev | AATGTGTAGCCTTTTTCAGGGAAAG |
babR-UTR-T7-For | TAATACGACTCACTATAGGGTAATCTTCGTAGAAGAAATATGAA |
babR-UTR-T7-Rev | TGTTTCCCATTTCATTGCAGAC |
babR-20-UTR-For | TAATACGACTCACTATAGGGTGAAAGATATGCTAGGAATGTTTAG |
rHfq-For | GCGGTCTCCGCGCATGGCTGAACGATCGCAAATCT |
rHfq-Rev | GCGGTCTCCTATCAGGCTTCCTCGCCTTCAAACAT |
rBabR-For | CATGAAATGGGAAACATTTTATG |
rBabR-Rev | AGGAGACCAAATGTACGGGCC |
PvirB-For | CTGACTGGGCGTCATTCACAC |
PvirB-Rev | CGTCTCCTTCTCAGAGAATGGACG |
babR-RACE-Rev | CAGGTCATGCGCATAATTCTTTACT |
Underlined sections indicate restriction endonuclease recognition sequences.