Table 2.
Plasmids used in this study
| Plasmid | Description | Reference |
|---|---|---|
| pUC19 | Cloning vector; Ampr | 54 |
| pGEM-T Easy | Cloning vector; Ampr | Promega |
| pEX100T | Cloning vector; contains sacB gene; Ampr | 38 |
| pBBR1MCS-4 | Broad-host-range cloning vector; Ampr | 20 |
| pNPTS138 | Cloning vector; contains sacB gene; Kanr | 46 |
| pLKC-481 | Cloning vector containing promoterless lacZ gene; Ampr Kanr | 48 |
| pMR10 | Broad-host-range cloning vector; Kanr | 14 |
| pMR15 | Broad-host-range vector containing promoterless lacZ gene; Kanr | 3 |
| pC3003 | virB-lacZ transcriptional fusion in pMR15 | This study |
| pC3004 | virB-lacZ translational fusion in pLKC-481 | This study |
| pC3005 | virB-lacZ translational fusion fragment from pC3004 in pMR10 | This study |
| pC3006 | babR-lacZ transcriptional fusion in pMR15 | This study |
| pC3007 | babR-lacZ translational fusion in pLKC-481 | This study |
| pC3008 | babR-lacZ translational fusion fragment from pC3007 in pMR10 | This study |
| pC3009 | In-frame deletion of babR plus 1 kb of each flanking region in pUC19 | This study |
| pC3010 | Mutated babR locus plus flanking regions from pC3009 in pNPTS138 | This study |
| pC3011 | babR locus including entire promoter region in pBBR1MCS-4 | This study |
| pC3012 | babR locus with truncated 5′ UTR in pUC19 | This study |
| pC3013 | babR-lacZ translational fusion with truncated 5′ UTR in pLKC-481 | This study |
| pC3014 | Truncated babR-lacZ translational fusion fragment from pC3013 in pMR10 | This study |
| pJMG001 | Full-length hfq gene plus 1 kb of each flanking region in pGEM-T Easy | This study |
| pJMG002 | Religation of pJMG001 following inverse PCR to delete hfq coding region | This study |
| pJMG003 | Mutated hfq locus plus flanking regions from pJMG002 in pEX100T | This study |
| pJMG004 | hfq locus including entire promoter region in pBBR1MCS-4 | This study |