Fig 5.

ppGpp accumulation in response to starvation in swarmer versus stalked cells. Mixed cultures were labeled with KH₂³²PO₄. The swarmer and stalked-cell bands were separated in a Percoll gradient, washed separately, and then resuspended in M2 defined medium lacking glucose. Samples were extracted, and nucleotides were resolved by TLC at time points after starvation (n = 6; linear regression; P < 0.01). Error bars represent standard errors of the means.