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Amino acid sequences of the β-sheet regions exchanged between KshA1 and KshA5 resulting in the chimeric enzymes KshA1A5β and KshA5A1β, respectively. The boxed amino acid residues were exchanged between KshA1 and KshA5, resulting in the chimeric enzymes KshA1A5loop, KshA1A5TG (italic), KshA5A1loop, KshA1A5β201-210, KshA5A1β207-216, KshA1A5β230-238, KshA5A1β236-244, KshA1D242W, and KshA5W248D. *, putative substrate-interacting residues (2).
