Table 4.
Molar ratios of KshA/KshBa and activity of (chimeric) enzymes with AD
| Chimeric KshA | Molar ratio of KshA/KshBb | Exptl Vmax ADb (nmol min−1 mg−1) |
|---|---|---|
| KshA1 | 1:1 ± 0.16 | 261 ± 11 |
| KshA5 | 1:0.59 ± 0.04 | 151 ± 30 |
| KshA1A5β | 1:3.16 ± 0.16 | 336 ± 27 |
| KshA5A1β | 1:1.43 ± 0.03 | 82 ± 11 |
| KshA1A5loop | 1:1.6 ± 0.1 | 366 ± 25 |
| KshA1A5TG | 1:1.27 ± 0.07 | 196 ± 24 |
| KshA5A1loop | 1:1 ± 0.01 | 248 ± 24 |
| KshA1A5β201-210 | 1:1.51 ± 0.11 | 415 ± 40 |
| KshA5A1β207-216 | 1:0.93 ± 0.04 | 421 ± 34 |
| KshA1D242W | 1:1.86 ± 0.04 | 125 ± 20 |
| KshA5W248D | 1:2.6 ± 0.3 | 231 ± 22 |
a
Determined by densitometry.
b
Error is standard error of the mean (SEM), n = 3.