Fig 4.

Correlation between the slower migrating FdnG polypeptide species identified in certain ATC trafficking mutants with that observed in a ΔtatC strain. In the interest of clarity, only the relevant genotypes of the strains analyzed are shown. Samples (50 μg protein) of crude extracts derived from strains LL402 (ΔerpA), JW2513 (ΔiscU), PB1000 (Δfnr) JW3815 (ΔtatC), DV1151 (ΔerpA ΔiscA), and MC4100 (wild type) after anaerobic growth in TGYEP medium (pH 6.5) with 100 mM nitrate were separated in SDS–8% (wt/vol) polyacrylamide gels, transferred to nitrocellulose membranes, and probed with antiserum raised against FdnG. The migration positions of the apoprotein form (apo-FdnG) and the processed species of FdnG are indicated to the left of the gel, and the sizes (in kilodaltons) of the molecular mass standards (PageRuler Prestained; Fermentas) are given to the right of the gel.