Table 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant genotype or phenotypea | Reference(s) or sourceb |
|---|---|---|
| E. coli strains | ||
| MC4100 | F−araD139 Δ(argF-lac)U169 ptsF25 deoC1 relA1 flbB5301 rspL150 | 10 |
| MG1655 | F− λ−ilvGrfb-50rph-1 | 9 |
| BW25113 | F− Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ−rph-1 Δ(rhaD-rhaB)568 hsdR514 | 4, 13 |
| CP477 | As MC4100 but ΔiscA::Kanr; ECK2525 | This study |
| CP1221 | As BW25113 but ΔsufA::cat | This study |
| CP1223 | As MC4100 but ΔsufA::cat | This study |
| PB1000 | As MC4100 but Δfnr | 35 |
| LL401 | MG1655 catRExBADerpA, erpA placed under the control of the pBAD promoter | 26 |
| LL402 | MG1655 ΔerpA::Cmr; ECK0155 | 26 |
| DV1151 | MG1655 ΔerpA::Cmr ΔiscA | 49 |
| JW2512 | BW25113 ΔiscA::Kanr; ECK2525 | NBP |
| JW2513 | BW25113 ΔiscU::Kanr; ECK2526 | NBP |
| JW1470 | BW25113 ΔfdnG::Kanr; ECK1468 | NBP |
| JW1215 | BW25113 ΔnarG::Kanr; ECK1218 | NBP |
| JW3815 | BW25113 ΔtatC::Kanr; ECK3832 | NBP |
| Plasmids | ||
| pCP20 | FLP+ λcI857+ λpR Repts Ampr Cmr | 12 |
| perpA | pBluescript SK(+) containing erpA in BamHI and EcoRI site; Ampr | This study |
| pLUE-A | pUC18 expressing erpA; Ampr | 26 |
| piscA | pBluescript SK(+) containing iscA in BamHI and EcoRI site; Ampr | This study |
| psufA | pBluescript SK(+) containing sufA in BamHI and EcoRI site; Ampr | This study |
a
Allele numbers are given for single gene mutants and refer to E. coli K-12 nomenclature.
b
NBP, National BioResources Project (NIG, Japan): E. coli.